DNA vector 1: p.gene of interest_Amp
Figure 1: My two DNA vectors. One has the green tag on at the end of the XX gene and the other doesn’t.
That’s what the correct plasmid should look like!
Figure 2: Agarose gel showing fragments of DNA after enzyme digestion from each tube (1-6).
After running multiple reactions to make other vector constructs as well I run a gel after digesting all 6 constructs with enzymes 1-3. Tube contains DNA Vector 2: p.gene of interest-GFP_Amp_RFP. This can now be put in the cells.
Which tube contained the right final vector we wanted (DNA vector 2: p.gene of interest-GFP_Amp_RFP)?